Analyzing the basophil count, the variation regarding room temperature and refrigeration was homogeneous during the whole storage period, not causing significant statistical difference Table 2. Significant variation was observed in platelet count. In this study, there was no statistical significance in erythrocyte count between groups K 2 EDTA and K 3 EDTA, and, after the 8-hour period, it was possible to observe neither quantity nor morphological alterations in cells.
When analyzing results obtained from samples at room temperature, we verified that hematocrit increased due to the increased volume of erythrocytes, demonstrating significant statistical variation. However, the samples under refrigeration kept their values stable. Determining the stability of complete blood count parameters in stored blood samples using the Sysmex XE automated haematology analyser.
Int J Lab Hematol. In the MCHC values of samples at room temperature, there was a slight oscillation after the fourth hour, returning to stability after the sixth hour. According to De Baca et al. Although oscillation in the obtained values of MCHC was observed, this difference did not occur in the values of hemoglobin concentration; there was stability in both anticoagulants, in all temperatures.
As for MCH, there was stability in results, without creating statistical relevance for samples during the analyzed period. The results of leukocyte count did not present significant statistical difference for either of the temperatures during the analyzed period.
It is known that leukocyte count can be altered in excess of EDTA due to sample dilution, affecting the differential count. The results of lymphocyte, neutrophil, and monocyte counts did not present statistical difference, but greater stability in refrigerated samples was observed.
Studies show that neutrophils and monocytes are more sensitive, and lymphocytes are more stable in storage with EDTA 4 4 Patel N. EDTA, the traditional anticoagulant of haematology: with increased automation is it time for a review? Med Lab Sci. The results obtained in platelet count presented a progressive decrease at room temperature, but without statistical difference, while refrigerated samples presented a considerable decrease in platelet count, with important statistical difference, which can have been caused by spontaneous agglutination.
Hemograma: como fazer e interpretar. Refrigerated samples did not suffer significant change in the parameters when in the analysis within an 8-hour period, except platelet analyses, in which there were relevant alterations.
Factors of the pre-analytical phase, such as temperature and storage time, can interfere with variability of blood count parameters in a clinical laboratory routine, and can produce mistaken results. According to the present study and several other results, we conclude that storing blood samples interferes with neither erythrocyte and leukocyte counts, nor hemoglobin concentration measurement.
However, it becomes unfeasible to assess other parameters after the 8-hour period, regardless of the storage form. One can also notice a marked decrease in platelet count, therefore we need to count and analyze coagulation parameters as soon as possible upon sample reception. Abrir menu Brasil. Jornal Brasileiro de Patologia e Medicina Laboratorial.
Abrir menu. The differences are the types of closures and the labeling. The pink stoppered tubes have blood bank labels and are generally sent to the blood bank laboratory in the hospital. The additive is the same in both tubes. The minimum and maximum acceptable blood volumes should be established by each facility, to ensure that accurate hematology results are obtained. BD Vacutainer blood collection tubes are designed to draw the appropriate volume to ensure a proper blood to additive ratio.
Learn More. Blood collection in heparin tubes for cytogenetic, and ethylenediaminetetraacetic acid EDTA tubes for molecular genetics applications respectively, are routine practices everywhere. If blood samples are required for cytogenetics as well as DNA work, two samples from each animal are usually collected, which leads to wastage of time and money. The present study tried to explore the possibilities of collecting a single blood sample in a heparinised tube for use in both applications.
Two blood samples were collected from the same animals; one in a heparin tube and the other in an EDTA tube. DNA was extracted and stored at the same temperature and for the same durations.
Comparative studies revealed that the DNA samples extracted from blood using these two different coagulants give more or less the same quality of results especially for polymerase chain reaction PCR based applications in cattle. The purpose of the present study was to establish the possibility of using heparin blood for chromosomal studies as well as for molecular biology. Such a practice will obviously save time and money in collecting samples in duplicate. Long term storage of DNA samples is required to create a genomic DNA bank for cattle and to minimize the future cost of research.
Successful long term storage depends on the stability of the DNA samples which in turn depends on storage conditions. DNA is an inherently stable molecule frequently used in molecular research. Research scientists utilizing blood in their studies have unique needs depending on their downstream applications. Blood can be collected using different anticoagulants including citrate, ethylenediaminetetraacetic acid EDTA or heparin. The type of anticoagulant used in blood collection can affect the results from blood DNA isolation, and may influence the results of research-based or diagnostic tests associated with blood.
Research scientists must ensure that their blood DNA isolation method is flexible, i. However, it does affect magnesium concentrations in downstream applications. Heparin should be avoided, as it can bind to DNA during purification and can inhibit Taq polymerase used for polymerase chain reaction PCR 1. In other words, sodium heparin, an anticoagulant used widely for blood collection, has been known to inhibit DNA polymerase activity in PCR assays 2. Irrespective of the anticoagulant, the vacutainer tube should be inverted several times to mix the blood.
Blood can be shipped at ambient temperature, but if the delay between collection and extraction is more than three days, there will be some degradation of DNA and the yield will be lower than that from fresh blood. In a healthy person, this process kicks into play almost immediately after sustaining damage to the endothelial tissue that lines blood vessels. A very common cause of this damage is venepuncture.
When damage occurs to a blood vessel, it constricts in order to reduce blood flow whilst circulating platelets stick to the site of injury. The formation of the platelet plug is known as primary haemostasis. Primary haemostasis is swiftly followed by secondary haemostasis - a clot made entirely of platelets is not robust enough to withstand the blood pressure in many blood vessels and so, various other plasma proteins are recruited to strengthen the plug.
In a series of interlinked enzyme reactions, these proteins help to make fibrin strands, which forms a strong and supportive mesh around the the platelet plug. This is why we sometimes observe clots in blood sample tubes.
It also preserves the blood sample to ensure that the constituents to be analysed are not significantly changed prior to the analytical process. With the correct blood sampling procedure, the collected blood is exposed to the EDTA which binds and withholds calcium ions thereby blocking the activation or progression of the coagulation cascade — ultimately inhibiting clot formation.
As mentioned earlier, primary haemostasis occurs immediately after venepuncture, so healthcare staff collecting samples must work promptly when collecting samples.
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